The lab uses a multifaceted approach to study the ubiquitination proteasome system (UPS) and its role in plant development. These include generation of transgenic plants, analysis of knockout lines, proteomics, gene expression analyses and protein interaction studies. The highly conserved UPS effectively controls the abundance of regulatory proteins, thus allowing for adaptation to external stimuli and regulation of growth and development. Targeting a specific protein for degradation by the 26S proteasome involves the covalent attachment of ubiquitin to the target in a conjugation cascade involving the enzymes, E1, E2 and E3. Research in the lab focuses on a large family of E3 ligases which are defined by the presence of a RING domain. Dr. Stone's group attempts to address the importance of these RING-type E3s in plant development by verifying ligase activity, identifying interacting proteins and determining biological function.

Hope you enjoy your visit!

Dr. Sophia Stone

BSc (York University, 1998)
PhD (York University, 2003)

Lab News

Date Item
06.06.2014 CONGRATULATIONS Sarah! Sarah recieved a NSERC CGS M. Well done.
05.06.2014 Welcome Celia! Celia will be working with us this summer. She was awarded a Sarah Lawson Scholarship to fund her research. Congrats Celia.
04.12.2014 Sophia was awarded the 2014 Killam Prize, congratulations.
01.05.2014 Welcome back Daryl. After the completeion of a successful honors project, Daryl is now doing research for his Masters thesis.
09.01.2013 Welcome Sarah! Sarah recently joined the lab as a Masters Student.
06.11.2013 New Paper! Chen YT, Liu H, Stone S, Callis J. (2013) ABA and the ubiquitin E3 ligase KEEP ON GOING affect proteolysis of the Arabidopsis thaliana transcription factors ABF1 and ABF3. Plant J. Jun 6. doi: 10.1111/tpj.12259. [Epub ahead of print]
05.31.2013 New Paper! Lyzenga WJ, Liu H, Schofield A, Muise-Hennessey A*, Stone SL. (2013) Arabidopsis CIPK26 interacts with KEG, components of the ABA signalling network and is degraded by the ubiquitin-proteasome system. J Exp Bot. 2013 May 8. * Undergaduate student
05.31.2013 New Paper! Liu H, Stone SL (2013) Cytoplasmic Degradation of the Arabidopsis Transcription Factor ABSCISIC ACID INSENSITIVE 5 is Mediated by the RING-type E3 Ligase KEEP ON GOING J. Biol. Chem. published May 29, 2013 as doi:10.1074/jbc.M113.46536
05.20.2013 Wendy will be attending Plant Biology 2013 on July 20-24 in Providence Rhode Island. Wendy will be giving an oral presentation on July 21st in Minisymposium 3: Signaling.

We will be attending the 2013 CSPB/SCBV Annual General Meeting on June 25-28 at the Univ. of Laval. Wendy will be giving a oral presentation and Daryl will be presenting a poster.

Congratulations Wendy! Winner of the CSPB/SCBV2013 Best Oral Presentation Award. Well done.


We will be attending the  9th annual Canadian Plant Genomics Workshop (CPGW) on August 12-15 in Halifax. Jiwon and Wendy wil be presenting posters. http://cpgw2013.org/

Congratulations Wendy! Winner of the CPGW Best Poster Presentation Award.

05.09.2013 CONGRATULATIONS to Sky, Daryl and Scott for successful competion of their Honors Thesis. Great work guys. All the best with grad school Sky and Scott. Daryl will be continuing in the lab as a Sarah Lawson award holder for the summer and then as a Masters student.
04.15.2013 CONGRATULATIONS to Daryl and Jiwon on recieveing a Sarah Lawson Award for summer research. Welcome to the lab Jiwon.
05.07.2012 New Paper! Lyzenga WJ, Booth JK*, Stone SL. (2012) The Arabidopsis RING-type E3 ligase XBAT32 mediates the proteasomal degradation of the ethylene biosynthetic enzyme, 1-aminocyclopropane-1-carboxylate synthase 7. Plant J. [Epub ahead of print] * Undergaduate student
05.03.2012 Wendy will be giving a talk at the 2012 CSPB Annual Meeting at the University of Alberta, June25-27th.
04.27.2012 CONGRATULATIONS to Alexandria, Will, Judith and Taq for sucessfull completeion of their Honor Thesis! Excellent Job. Good luck with grad school Will.